The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata
	Liu, Yangjia; Zhang, Rongqing; Xie, Liping; Zhan, Aibin; Huang, Jingliang; Li, Shiguo
刊名	SCIENTIFIC REPORTS
	2017-10-01
卷号	7 期号:0 页码:-
英文摘要	Mounting evidence suggests that TGF beta/BMP signaling pathway is most likely involved in shell biomineralization in molluscs, but the function of pathway receptors is poorly studied. Here, we cloned and identified two homologous BMP receptor genes, PfBMPR1B and PfBAMBI, from the pearl oyster Pinctada fucata. Real-time quantitative PCR and in situ hybridization revealed that these genes were expressed in mantle edge and pallial, specifically located at the outer epithelia. Knockdown of PfBMPR1B by RNA interference ( RNAi) significantly decreased the expression levels of matrix protein ( MP) genes and induced the abnormal ultrastructure of prismatic and nacreous layers. Conversely, knockdown of PfBAMBI significantly increased the expression levels of a portion of MP genes and induced the overgrowth of nacreous layer crystals. In the RNAi and shell notching experiments, MP gene expressions were competitively regulated by PfBMPR1B and PfBAMBI. In addition, the receptor inhibitor LDN193189 reduced the expression levels of MP genes in mantle primary cells and larvae, and induced abnormal D-shaped shell formation during larval development. Collectively, these results clearly show that PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in P. fucata. Our study therefore provides the direct evidence that BMP receptors participate in mollusc biomineralization.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39435]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liu, Yangjia,Zhang, Rongqing,Xie, Liping,et al. The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata[J]. SCIENTIFIC REPORTS,2017,7(0):-.
APA	Liu, Yangjia,Zhang, Rongqing,Xie, Liping,Zhan, Aibin,Huang, Jingliang,&Li, Shiguo.(2017).The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata.SCIENTIFIC REPORTS,7(0),-.
MLA	Liu, Yangjia,et al."The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata".SCIENTIFIC REPORTS 7.0(2017):-.