Microgravity-induced hepatogenic differentiation of rBMSCs on board the SJ-10 satellite

doi:10.1096/fj.201802075R

	Microgravity-induced hepatogenic differentiation of rBMSCs on board the SJ-10 satellite
	Lv DY(吕东媛); Sun SJ(孙树津); Zhang F(张帆); Luo CH(罗春花); Zheng L ; Wu Y(武毅); Li N(李宁); Zhang C; Wang CZ; Chen Q
刊名	FASEB JOURNAL
	2019-03-01
卷号	33 期号:3 页码:4273-4286
关键词	mesenchymal stem cells mechanotransduction hepatocyte signaling pathway
ISSN号	0892-6638
DOI	10.1096/fj.201802075R
英文摘要	Bone marrow-derived mesenchymal stem cells (BMSCs) are able to differentiate into functional hepatocytelike cells, which are expected to serve as a potential cell source in regenerative medicine, tissue engineering, and clinical treatment of liver injury. Little is known about whether and how space microgravity is able to direct the hepatogenic differentiation of BMSCs in the actual space microenvironment. In this study, we examined the effects of space microgravity on BMSC hepatogenic differentiation on board the SJ-10 Recoverable Scientific Satellite. Rat BMSCs were cultured and induced in hepatogenic induction medium for 3 and 10 d in custom-made space cell culture hardware. Cell growth was monitored periodically in orbit, and the fixed cells and collected supernatants were retrieved back to the Earth for further analyses. Data indicated that space microgravity improves the differentiating capability of the cells by up-regulating hepatocyte-specific albumin and cytokeratin 18. The resulting cells tended to be maturated, with an in-orbit period of up to 10 d. In space, mechanosensitive molecules of 1-integrin, -actin, -tubulin, and Ras homolog gene family member A presented enhanced expression, whereas those of cell-surface glycoprotein CD44, intercellular adhesion molecule 1, vascular cell adhesion molecule 1, vinculin, cell division control protein 42 homolog, and Rho-associated coiled-coil kinase yielded reduced expression. Also observed in space were the depolymerization of actin filaments and the accumulation of microtubules and vimentin through the altered expression and location of focal adhesion complexes, Rho guanosine 5'-triphosphatases, as well as the enhanced exosome-mediated mRNA transfer. This work furthers the understanding of the underlying mechanisms of space microgravity in directing hepatogenic differentiation of BMSCs.Lu, D., Sun, S., Zhang, F., Luo, C., Zheng, L., Wu, Y., Li, N., Zhang, C., Wang, C., Chen, Q., Long, M. Microgravity-induced hepatogenic differentiation of rBMSCs on board the SJ-10 satellite.
分类号	二类/Q1
WOS关键词	MESENCHYMAL STEM-CELLS ; SIMULATED MICROGRAVITY ; OSTEOGENIC DIFFERENTIATION ; ENDOTHELIAL-CELLS ; LIVER ; HEPATOCYTES ; EXPRESSION ; GRAVITY ; ACTIVATION ; CAPACITY
WOS研究方向	Biochemistry & Molecular Biology ; Biology ; Cell Biology
语种	英语
WOS记录号	WOS:000459794800094
资助机构	National Natural Science Foundation of China [U1738115, 31661143044] ; Strategic Priority Research Program ; Frontier Science Key Project of Chinese Academy of Sciences [XDA0402020219, XDA04020416, XDA04073800, QYZDJSSW-JSC018]
其他责任者	Long, M
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/78431]
专题	力学研究所_国家微重力实验室
作者单位	1.{Lu, Dongyuan、Sun, Shujin、Zhang, Fan、Luo, Chunhua、Zheng, Lu、Wu, Yi、Li, Ning、Zhang, Chen、Wang, Chengzhi、Chen, Qin、Long, Mian} Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing, Peoples R China 2.{Lu, Dongyuan、Sun, Shujin、Zhang, Fan、Luo, Chunhua、Zheng, Lu、Wu, Yi、Li, Ning、Zhang, Chen、Wang, Chengzhi、Chen, Qin、Long, Mian} Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing, Peoples R China 3.{Lu, Dongyuan、Sun, Shujin、Zhang, Fan、Zheng, Lu、Wu, Yi、Li, Ning、Wang, Chengzhi、Long, Mian} Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Lv DY,Sun SJ,Zhang F,et al. Microgravity-induced hepatogenic differentiation of rBMSCs on board the SJ-10 satellite[J]. FASEB JOURNAL,2019,33(3):4273-4286.
APA	吕东媛.,孙树津.,张帆.,罗春花.,Zheng L.,...&龙勉.(2019).Microgravity-induced hepatogenic differentiation of rBMSCs on board the SJ-10 satellite.FASEB JOURNAL,33(3),4273-4286.
MLA	吕东媛,et al."Microgravity-induced hepatogenic differentiation of rBMSCs on board the SJ-10 satellite".FASEB JOURNAL 33.3(2019):4273-4286.