Real-Time Dissecting the Entry and Intracellular Dynamics of Single Reovirus Particle

doi:10.3389/fmicb.2018.02797

CORC > 武汉植物园 > 中国科学院武汉植物园

	Real-Time Dissecting the Entry and Intracellular Dynamics of Single Reovirus Particle
	Zhang, Qi-Ya 2; Pang, Dai-Wen 1; Gui, Jian-Fang 2; Yu, Cong 1; Liu, Jia 2
刊名	FRONTIERS IN MICROBIOLOGY
	2018-11-20
卷号	9 页码:17
关键词	non-enveloped virus reovirus entry real-time single-particle tracking clathrin cytoskeletons endosomes
ISSN号	1664-302X
DOI	10.3389/fmicb.2018.02797
英文摘要	Reoviruses are non-enveloped viruses with wide host range, can cause serious infections in animals, plants and microorganism, e.g., aquareovirus, which is capable of causing serious haemorrhagic in aquatic animals. To date, the entry process of aquareovirus infection remains obscure. Real-time single-virus tracking are effective tools for exploring the details in viral infection process, which are crucial for understanding the pathogenic mechanism. Here, we used quantum dots-based single particle tracking technology combined with biochemical assays and ultrastructural observation to reveal unobservable infection steps and map dynamic interactions between a reovirus, Scophthalmus maximus reovirus (SMReV), and its host cell in real time. The results showed that the single membrane-bound reovirus particle can enter into the cell within several seconds through nascent clathrin-caoted pits, and most of the particles could internalize into cytoplasm within 30 min post-infection. The specific inhibitors analysis also showed that entry of SMREV depended on clathrin-mediated endocytosis rather than cavolin-mediated endocytosis. The motion analysis of internalized single particle indicated that the reovirus initially experienced slow and directed motion in the actin-enriched cell periphery, while it underwent relatively faster and directed movement toward the cell interior, suggesting that transport of SMReV was dependent on the cytoskeleton. Further, dual-labeling of virus and cytoskeleton and inhibitor analysis both demonstrated that transport of internalized SMReV was firstly dependent on actin filaments at the cell periphery, and then on microtubules toward the cell interior. Then visualization of SMReV trafficking in the endosomes revealed that the internalized reovirus particles were sorted from early endosomes to late endosomes, then part of them were delivered to lysosome. This study for the first time revealed the entry pathway, intracellular dynamic and the infection fate of fish reovirus in host cell in real time and in situ, which provided new insights into the infection mechanism of non-enveloped viruses.
资助项目	National Natural Science Foundation of China[31430091] ; National Natural Science Foundation of China[31772890] ; Strategic pilot science and technology of the Chinese Academy of Sciences project[XDA08030202] ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01]
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000450669800001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/6121]
专题	中国科学院武汉植物园
通讯作者	Zhang, Qi-Ya
作者单位	1.Wuhan Univ, Key Lab Analyt Chem Biol & Med, Coll Chem & Mol Sci, Minist Educ,State Key Lab Virol,Inst Adv Studies, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qi-Ya,Pang, Dai-Wen,Gui, Jian-Fang,et al. Real-Time Dissecting the Entry and Intracellular Dynamics of Single Reovirus Particle[J]. FRONTIERS IN MICROBIOLOGY,2018,9:17.
APA	Zhang, Qi-Ya,Pang, Dai-Wen,Gui, Jian-Fang,Yu, Cong,&Liu, Jia.(2018).Real-Time Dissecting the Entry and Intracellular Dynamics of Single Reovirus Particle.FRONTIERS IN MICROBIOLOGY,9,17.
MLA	Zhang, Qi-Ya,et al."Real-Time Dissecting the Entry and Intracellular Dynamics of Single Reovirus Particle".FRONTIERS IN MICROBIOLOGY 9(2018):17.