Influence of patterned titanium coatings on polarization of macrophage and osteogenic differentiation of bone marrow stem cells

doi:10.1177/0885328217746802

	Influence of patterned titanium coatings on polarization of macrophage and osteogenic differentiation of bone marrow stem cells
	Zhang, Zequan 1; Xie, Youtao ; Pan, Houhua ; Huang, Liping ; Zheng, Xuebin
刊名	JOURNAL OF BIOMATERIALS APPLICATIONS
	2018
卷号	32 期号:7 页码:977
关键词	Osteoimmunomodulatory properties macrophage E-cadherin pattern titanium coating
ISSN号	0885-3282
DOI	10.1177/0885328217746802
英文摘要	Biomaterial surface topography plays a vital role in the osteointegration of implants by regulating the early cell responses and tissue growth-in. However, most of the previous researches focused on the effects of osteogenic cells, only a little is known about the immune cells which dominate osteogenesis after implanting. In this paper, patterned titanium coatings were fabricated and the effects of surface topography on the macrophage behaviors were investigated. On patterned titanium surface, macrophages preferred to polarize to M2, while macrophages on traditional titanium coatings presented higher M1 polarization. Nearly 70% higher expression of anti-inflammatory genes, including interleukin-4, interleukin-10, interleukin-1ra, and arginase, were detected on the patterned titanium coatings. While the pro-inflammatory genes, such as interleukin-1, interleukin-6, tumor necrosis factor-, interferon-, and inducible nitric oxide synthase were notably depressed. Up-regulation of the osteoinductive cytokines were also detected on the patterned coatings, which indicated advantageous osteogenic microenvironment provided by macrophages. Immunomodulation effect on osteogenesis was also investigated in this study. Stimulated with RAW cells/patterned coatings conditioned medium, bone marrow stem cells presented nearly 1.5 fold higher expression of osteogenic genes and more mineralization nodules than the traditional sprayed Ti coatings. All these results suggested that modulating materials with a patterned surface might be a valuable strategy to endow the implants with favorable osteoimmunomodulatory properties.
学科主题	Engineering, Biomedical ; Materials Science, bioMaterials
出版者	SAGE PUBLICATIONS LTD
WOS记录号	WOS:000419897400013
资助机构	The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: the National Natural Science Foundation of China (Grant No. 51502328). ; The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: the National Natural Science Foundation of China (Grant No. 51502328).
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/24519]
专题	中国科学院上海硅酸盐研究所
作者单位	1.Chinese Acad Sci, Shanghai Inst Ceram, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zequan,Xie, Youtao,Pan, Houhua,et al. Influence of patterned titanium coatings on polarization of macrophage and osteogenic differentiation of bone marrow stem cells[J]. JOURNAL OF BIOMATERIALS APPLICATIONS,2018,32(7):977, 986.
APA	Zhang, Zequan,Xie, Youtao,Pan, Houhua,Huang, Liping,&Zheng, Xuebin.(2018).Influence of patterned titanium coatings on polarization of macrophage and osteogenic differentiation of bone marrow stem cells.JOURNAL OF BIOMATERIALS APPLICATIONS,32(7),977.
MLA	Zhang, Zequan,et al."Influence of patterned titanium coatings on polarization of macrophage and osteogenic differentiation of bone marrow stem cells".JOURNAL OF BIOMATERIALS APPLICATIONS 32.7(2018):977.