Evaluation of 10 plant barcodes in Bryophyta (Mosses)

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	Evaluation of 10 plant barcodes in Bryophyta (Mosses)
	Liu, Y ; Yan, HF ; Cao, T ; Ge, XJ
刊名	JOURNAL OF SYSTEMATICS AND EVOLUTION
	2010
卷号	48 期号:1 页码:36-46
关键词	Bryophyta DNA barcoding rbcL rpoC1 rps4 trnH-psbA trnL-trnF
ISSN号	0529-1526
通讯作者	Cao, T, Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China
中文摘要	DNA barcoding is a molecular tool that uses a standardized DNA region to identify species. Our preliminary study reported here is the first attempt to specifically focus on universality and attributes of candidate barcodes across a wide systematic range of mosses. We tested eight previously proposed plant barcoding regions (atpf-atpH, ITS2, matK, psbK-psbI, rbcL, rpoB, rpoC1, and trnH-psbA) and two popular phylogenetic markers (rps4 and trnL-trnF of cpDNA) in 49 moss species and 9 liverwort species, representing half of the orders in moss lineages. The ITS2, rbcL, rpoC1, rps4, trnH-psbA and trnL-trnF regions showed good universality, and therefore the efficacy of these loci as DNA barcodes was further evaluated in 36 mosses and 2 liverworts, each of which included two to three individuals per taxa. The five loci, viz. rbcL, rpoC1, rps4, trnH-psbA and trnL-trnF, were easy to amplify and sequence and showed significant inter-specific genetic variability, making them potentially useful DNA barcodes for mosses. The best performing single loci were the rbcL and rpoC1 coding regions. Several loci showed equivalent performance and combinations of them did not greatly increase their discrimination capacity. In addition, phylogenies generated from each of the separate regions and multi-locus combinations by using best-fit and Kimura 2-parameter models were compared, but no significant difference was found.
学科主题	Plant Sciences
收录类别	SCI
资助信息	Chinese Academy of Sciences [2009-LSF-GBOWS-01]; Natural Science Foundation of China [30770141]; Shanghai Leading Academic Discipline Project [S30406]
语种	英语
WOS记录号	WOS:000274470600002
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/53923]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Liu, Y,Yan, HF,Cao, T,et al. Evaluation of 10 plant barcodes in Bryophyta (Mosses)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2010,48(1):36-46.
APA	Liu, Y,Yan, HF,Cao, T,&Ge, XJ.(2010).Evaluation of 10 plant barcodes in Bryophyta (Mosses).JOURNAL OF SYSTEMATICS AND EVOLUTION,48(1),36-46.
MLA	Liu, Y,et al."Evaluation of 10 plant barcodes in Bryophyta (Mosses)".JOURNAL OF SYSTEMATICS AND EVOLUTION 48.1(2010):36-46.