Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli

doi:10.1039/c7ra09519h

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli
	Xu, Xin 1,2; Xian, Mo 1; Liu, Huizhou 1
刊名	RSC ADVANCES
	2017
卷号	7 期号:80 页码:50942-50948
DOI	10.1039/c7ra09519h
文献子类	Article
英文摘要	Phloroglucinol, an important fine chemical, was attempted to be produced by a recombinant Escherichia coli, using acetate, a less costly feedstock, as a alternative carbon source. Phloroglucinol was significantly produced by assembling an acetate assimilation pathway and phloroglucinol biosynthetic pathway in an engineered Escherichia coli strain. Subsequently, the culture conditions were optimized to enhance phloroglucinol production with a maximum titer of 554 mg L-1. Finally, fed-batch fermentation of phloroglucinol was evaluated using the optimized culture conditions, and reached a maximum concentration of 1.20 g L-1. The productivity (0.74 g per g DCW) and yield (0.18 g per g acetate) increased by 3.20-fold and 1.64-fold, respectively, compared with the data using glucose as the carbon source. Therefore, the engineered E. coli cells can be directly emplored for phloroglucinol biosynthesis from acetate with better atom economy and lower cost.
WOS关键词	GENE-EXPRESSION ; ACETIC-ACID ; FERMENTATION ; OPTIMIZATION ; BIOSYNTHESIS ; TEMPERATURE ; BACTERIA ; PROTEIN
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000414405800064
资助机构	National Natural Science Foundation of China(21572242 ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; 21376255)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9689]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xin,Xian, Mo,Liu, Huizhou. Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli[J]. RSC ADVANCES,2017,7(80):50942-50948.
APA	Xu, Xin,Xian, Mo,&Liu, Huizhou.(2017).Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli.RSC ADVANCES,7(80),50942-50948.
MLA	Xu, Xin,et al."Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli".RSC ADVANCES 7.80(2017):50942-50948.