High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli

doi:10.1016/j.procbio.2017.07.021

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli
	Liu, Hui 1; Cheng, Tao 1; Zou, Huibin 1,2; Zhang, Haibo 1; Xu, Xin 1; Sun, Chao 1; Aboulnaga, Elhussiny 1,3; Cheng, Zhongkai 4; Zhao, Guang 1; Xian, Mo 1
刊名	PROCESS BIOCHEMISTRY
	2017-11-01
卷号	62 页码:1-9
关键词	Mevalonate Isoprene Sabinene Betaine Biosynthesis
ISSN号	1359-5113
DOI	10.1016/j.procbio.2017.07.021
文献子类	Article
英文摘要	Isoprenoids are important fine chemicals as material monomers, advanced fuels and pharmaceuticals. A variety of natural isoprenoids can be synthesized by engineered microbial strains. This work established a process by dividing the current isoprenoid pathway into the upstream fermentation process, from sugar to mevalonate (MVA), and the downstream process, from MVA to the target isoprenoids. The results showed that significant differences existed in the process conditions between the upstream and downstream fermentations. After individually optimizing the process conditions, the upstream MVA production (84.0 g/L, 34.0% and 1.8 g/ L/h) and downstream isoprene production (11.0 g/L and 0.23 g/L/h) were greatly improved in this two-step process. Flask fermentation experiments also confirmed that two-step route can significantly improve the sabinene titer to 150 mg/L (6.5-fold of the sabinene titer in an earlier flask study of our lab). Therefore, the two-step route proposed in this study may have potential benefits towards the current isoprenoids production directly from glucose. The high titer and yield of MVA indicate that MVA has great potential to be more broadly utilized as starting precursor in synthetic biology.
WOS关键词	BACILLUS-SUBTILIS ; ISOPENTENOL PRODUCTION ; SYNTHETIC BIOLOGY ; PATHWAY ; STRAIN ; IDENTIFICATION ; OPTIMIZATION ; SYNTHASES ; MICROBES ; GENES
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000416394500001
资助机构	Shandong Province Natural Science Foundation(ZR2015BM011) ; Technology Development Project of Shandong Province(2016GSF121013) ; National Natural Science Foundation(21106170 ; 100 Talent Project of CAS ; 21376129 ; 21572242 ; 31670493)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10483]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
通讯作者	Zou, Huibin; Zhao, Guang; Xian, Mo
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China 3.Mansoura Univ, Fac Agr, Mansoura 35516, Egypt 4.Jilin Univ, Sch Life Sci, Changchun, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hui,Cheng, Tao,Zou, Huibin,et al. High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli[J]. PROCESS BIOCHEMISTRY,2017,62:1-9.
APA	Liu, Hui.,Cheng, Tao.,Zou, Huibin.,Zhang, Haibo.,Xu, Xin.,...&Xian, Mo.(2017).High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli.PROCESS BIOCHEMISTRY,62,1-9.
MLA	Liu, Hui,et al."High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli".PROCESS BIOCHEMISTRY 62(2017):1-9.