cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis
	Wang, Chunyan 1,2; Zhao, Jianmin2 ; Mu, Changkao 1; Wang, Qing2 ; Wu, Huifeng2 ; Wang, Chunlin 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-02-01
卷号	34 期号:2 页码:697-703
关键词	Mytilus Galloprovincialis Molecular Cloning Glutathione S-transferases Innate Immune
ISSN号	1050-4648
通讯作者	Mu, CK (reprint author), Ningbo Univ, Sch Marine Sci, 818 Fenghua Rd, Ningbo 315211, Zhejiang, Peoples R China. muchangkao@nbu.edu.cn ; wangchunlin@nbu.edu.cn
产权排序	[Wang, Chunyan; Mu, Changkao; Wang, Chunlin] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; [Wang, Chunyan; Zhao, Jianmin; Wang, Qing; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
文献子类	Article
英文摘要	Glutathione S-transferases (GSTs) are phase II enzymes involved in the regulation of redox homeostasis and innate immune responses against bacterial infection, which also play important roles in the detoxification of xenobiotics. In this study, we reported four genes of the GST family (named MgGST alpha, MgGSTS1, MgGSTS2, and MgGSTS3, respectively) from Mytilus galloprovincialis. MgGST alpha, MgGSTS1, MgGSTS2, and MgGSTS3 consisted of open reading frame (ORF) of 648 bp, 612 bp, 621 bp and 609 bp respectively, which encoded proteins of 216, 204, 207 and 203 amino acids residues, respectively. Sequence analysis showed that the predicted protein sequence of MgGSTs contained the conserved domain of the GST_N and GST C. Alignment analysis indicated that the MgGSTs were divided into two types, one was of alpha GST, and the others were of sigma class. Tissue distribution study revealed that MgGST alpha, MgGSTS2, MgGSTS3 transcripts were highly expressed in hemocytes, while MgGSTS1 mRNA was most abundantly expressed in hepatopancreas. After bacterial challenge, the expression level of these MgGSTs in hemocytes were all significantly higher than that of the control group. These results suggested that MgGSTs might play important roles in the modulation of immune response in M. galloprovincialis. (C) 2012 Published by Elsevier Ltd.; Glutathione S-transferases (GSTs) are phase II enzymes involved in the regulation of redox homeostasis and innate immune responses against bacterial infection, which also play important roles in the detoxification of xenobiotics. In this study, we reported four genes of the GST family (named MgGST alpha, MgGSTS1, MgGSTS2, and MgGSTS3, respectively) from Mytilus galloprovincialis. MgGST alpha, MgGSTS1, MgGSTS2, and MgGSTS3 consisted of open reading frame (ORF) of 648 bp, 612 bp, 621 bp and 609 bp respectively, which encoded proteins of 216, 204, 207 and 203 amino acids residues, respectively. Sequence analysis showed that the predicted protein sequence of MgGSTs contained the conserved domain of the GST_N and GST C. Alignment analysis indicated that the MgGSTs were divided into two types, one was of alpha GST, and the others were of sigma class. Tissue distribution study revealed that MgGST alpha, MgGSTS2, MgGSTS3 transcripts were highly expressed in hemocytes, while MgGSTS1 mRNA was most abundantly expressed in hepatopancreas. After bacterial challenge, the expression level of these MgGSTs in hemocytes were all significantly higher than that of the control group. These results suggested that MgGSTs might play important roles in the modulation of immune response in M. galloprovincialis. (C) 2012 Published by Elsevier Ltd.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
WOS关键词	MOLECULAR-CLONING ; VENERUPIS-PHILIPPINARUM ; PI-CLASS ; MU-CLASS ; IDENTIFICATION ; HEMOCYTES ; SEQUENCES ; OYSTER ; CLAM
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000315076100035
资助机构	National Key Technology Support Program [2011BAD13B0903]; K.C. Wong Magna Fund
公开日期	2013-08-15
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6603]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chunyan,Zhao, Jianmin,Mu, Changkao,et al. cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):697-703.
APA	Wang, Chunyan,Zhao, Jianmin,Mu, Changkao,Wang, Qing,Wu, Huifeng,&Wang, Chunlin.(2013).cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis.FISH & SHELLFISH IMMUNOLOGY,34(2),697-703.
MLA	Wang, Chunyan,et al."cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):697-703.