Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics

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	Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics
	Liu, Xiaoli 1,2; Zhang, Linbao 1,2; You, Liping 1,2; Yu, Junbao1 ; Cong, Ming1 ; Wang, Qing1 ; Li, Fei1 ; Li, Lianzhen1 ; Zhao, Jianmin1 ; Li, Chenghua 1
刊名	CLEAN-SOIL AIR WATER
	2011-08-01
卷号	39 期号:8 页码:759-766
关键词	Bioindicator Manila Clam Mercury Metabolomics Nmr
ISSN号	1863-0650
通讯作者	Wu, HF, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
产权排序	[Liu, XL; Zhang, LB; You, LP; Yu, JB; Cong, M; Wang, Q; Li, F; Li, LZ; Zhao, JM; Li, CH; Wu, HF] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China; [Liu, XL; Zhang, LB; You, LP] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
文献子类	Article
英文摘要	There are mainly distributed three pedigrees (White, Liangdao Red, and Zebra) of Manila clam Ruditapes philippinarum in Yantai population along the Bohai marine and coast. However, the biological differences to environmental stressors have been ignored in toxicology studies, which could lead to the distortion of biological interpretations of toxicological effects induced by environmental contaminants. In this study, we applied a system biology approach, metabolomics to compare the metabolic profiles in digestive gland from three pedigrees of clam and characterize and compare the metabolic responses induced by mercury in clam digestive gland tissues to determine a sensitive pedigree of clam as a preferable bioindicator for metal pollution monitoring and toxicology research. The most abundant metabolites, respectively, included branched-chain amino acids, alanine, and arginine in White samples, glutamate, dimethylglycine, and glycine in Zebra clams and acetylcholine, betaine, glucose, and glycogen in Liangdao Red clams. After 48 h exposure of 20 mu g L(-1) Hg(2+), the metabolic profiles from the three pedigrees of clams showed differentially significant changes in alanine, glutamate, succinate, taurine, hypotaurine, glycine, arginine, glucose, etc. Our findings indicate the toxicological effects of mercury exposure in Manila clams including the neurotoxicity, disturbances in energetic metabolisms and osmoregulation in the digestive glands and suggest that Liangdao Red pedigree of clam could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive classes of metabolic changes from digestive glands compared with other two (White and Zebra) pedigrees of clams.; There are mainly distributed three pedigrees (White, Liangdao Red, and Zebra) of Manila clam Ruditapes philippinarum in Yantai population along the Bohai marine and coast. However, the biological differences to environmental stressors have been ignored in toxicology studies, which could lead to the distortion of biological interpretations of toxicological effects induced by environmental contaminants. In this study, we applied a system biology approach, metabolomics to compare the metabolic profiles in digestive gland from three pedigrees of clam and characterize and compare the metabolic responses induced by mercury in clam digestive gland tissues to determine a sensitive pedigree of clam as a preferable bioindicator for metal pollution monitoring and toxicology research. The most abundant metabolites, respectively, included branched-chain amino acids, alanine, and arginine in White samples, glutamate, dimethylglycine, and glycine in Zebra clams and acetylcholine, betaine, glucose, and glycogen in Liangdao Red clams. After 48 h exposure of 20 mu g L-1 Hg2+, the metabolic profiles from the three pedigrees of clams showed differentially significant changes in alanine, glutamate, succinate, taurine, hypotaurine, glycine, arginine, glucose, etc. Our findings indicate the toxicological effects of mercury exposure in Manila clams including the neurotoxicity, disturbances in energetic metabolisms and osmoregulation in the digestive glands and suggest that Liangdao Red pedigree of clam could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive classes of metabolic changes from digestive glands compared with other two (White and Zebra) pedigrees of clams.
学科主题	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
WOS关键词	LAGOON-OF-VENICE ; TAPES-PHILIPPINARUM ; MYTILUS-GALLOPROVINCIALIS ; H-1-NMR METABOLOMICS ; PATTERN-RECOGNITION ; MANILA CLAM ; SPECTROSCOPY ; METABONOMICS ; EXTRACTION ; POLLUTION
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
语种	英语
WOS记录号	WOS:000294720700007
资助机构	Chinese Academy of Sciences; Technology Development Program Projects of Shandong Province[2008GG20005006, 2008GG3NS0700]; SDSFC[ZR2009CZ008]; CAS/SAFEA
公开日期	2011-11-11
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5118]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_滨海湿地实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics[J]. CLEAN-SOIL AIR WATER,2011,39(8):759-766.
APA	Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Yu, Junbao.,Cong, Ming.,...&Wu, Huifeng.(2011).Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics.CLEAN-SOIL AIR WATER,39(8),759-766.
MLA	Liu, Xiaoli,et al."Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics".CLEAN-SOIL AIR WATER 39.8(2011):759-766.