Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 污染过程与控制实验室

	Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum
	Zhao, Jianmin1 ; Qiu, Lihua 2; Ning, Xuanxuan 3; Chen, Aiqin 1; Wu, Huifeng1 ; Li, Chenghua 1
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2010-05-01
卷号	156 期号:1 页码:56-60
关键词	Venerupis Philippinarum I-type Lysozyme Tissue Expression Time-course Expression
ISSN号	1096-4959
通讯作者	Li, CH, Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China
产权排序	[Zhao, Jianmin; Chen, Aiqin; Wu, Huifeng; Li, Chenghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Qiu, Lihua] Chinese Acad Fishery Sci, S China Sea Fishery Res Inst, Guangzhou 510301, Guangdong, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
文献子类	Article
英文摘要	Lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections and providing nutrition as digestion enzymes. In the present study, an invertebrate type lysozyme (denoted as VpLYZ) was identified from Venerupis philippinarum haemocytes by cDNA library and RACE approaches. The full-length cDNA of VpLYZ consisted of 805 nucleotides with a canonical polyadenylation signal sequence AATAAA and a polyA tail, and an open-reading frame of 558 bp encoding a polypeptide of 185 amino acids with a calculated molecular mass of 20.87 kD and theoretical pl of 8.44. The high similarity of VpLYZ with other i-type lysozymes from mollusk indicated that VpLYZ should be a new member of i-type lysozyme family. Similar to most i-type lysozymes, VpLYZ possessed all conserved features critical for the fundamental structure and function of i-type lysozymes, such as three catalytic residues (Glu19, Asn72 and Ser75) and i-type specific motif CL(E/L/R/H)C(I/M)C. By semi-quantitative RT-PCR analysis, mRNA transcript of VpLYZ was found to be most abundantly expressed in the tissues of gills, hepatopancreas and haemocytes, weakly expressed in the tissues of muscle, foot and mantle. After clams were challenged by Vibrio anguillarum, the mRNA level of VpLYZ in overall haemocyte population was recorded by quantitative real-time RT-PCR. VpLYZ mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level increased to the peak at 72 h and recovered to the original level at 96 h. All these results indicated that VpLYZ was involved in the immune response against microbe infection and contributed to the clearance of bacterial pathogens. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.; Lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections and providing nutrition as digestion enzymes. In the present study, an invertebrate type lysozyme (denoted as VpLYZ) was identified from Venerupis philippinarum haemocytes by cDNA library and RACE approaches. The full-length cDNA of VpLYZ consisted of 805 nucleotides with a canonical polyadenylation signal sequence AATAAA and a polyA tail, and an open-reading frame of 558 bp encoding a polypeptide of 185 amino acids with a calculated molecular mass of 20.87 kD and theoretical pl of 8.44. The high similarity of VpLYZ with other i-type lysozymes from mollusk indicated that VpLYZ should be a new member of i-type lysozyme family. Similar to most i-type lysozymes, VpLYZ possessed all conserved features critical for the fundamental structure and function of i-type lysozymes, such as three catalytic residues (Glu19, Asn72 and Ser75) and i-type specific motif CL(E/L/R/H)C(I/M)C. By semi-quantitative RT-PCR analysis, mRNA transcript of VpLYZ was found to be most abundantly expressed in the tissues of gills, hepatopancreas and haemocytes, weakly expressed in the tissues of muscle, foot and mantle. After clams were challenged by Vibrio anguillarum, the mRNA level of VpLYZ in overall haemocyte population was recorded by quantitative real-time RT-PCR. VpLYZ mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level increased to the peak at 72 h and recovered to the original level at 96 h. All these results indicated that VpLYZ was involved in the immune response against microbe infection and contributed to the clearance of bacterial pathogens. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Zoology
URL标识	查看原文
WOS关键词	GOOSE-TYPE LYSOZYME ; MARINE-INVERTEBRATES ; RECOMBINANT PROTEIN ; ADAPTIVE EVOLUTION ; MOLECULAR-CLONING ; CRASSOSTREA-GIGAS ; GENE-EXPRESSION ; ASTERIAS-RUBENS ; LYTIC ACTIVITY ; CDNA CLONING
WOS研究方向	Biochemistry & Molecular Biology ; Zoology
语种	英语
WOS记录号	WOS:000276734500009
资助机构	South China Sea Fishery Research Institute, Chinese Academy of Fishery Sciences and Chinese Academy of Sciences [kzcx2-yw-225, HK0810BX-049]; NSFC [30901115]; SDSFC [ZR2009CZ008]
公开日期	2011-07-14
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/3782]
专题	烟台海岸带研究所_污染过程与控制实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Fishery Sci, S China Sea Fishery Res Inst, Guangzhou 510301, Guangdong, Peoples R China 3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jianmin,Qiu, Lihua,Ning, Xuanxuan,et al. Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,156(1):56-60.
APA	Zhao, Jianmin,Qiu, Lihua,Ning, Xuanxuan,Chen, Aiqin,Wu, Huifeng,&Li, Chenghua.(2010).Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,156(1),56-60.
MLA	Zhao, Jianmin,et al."Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 156.1(2010):56-60.