A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase

doi:10.1007/s41365-016-0157-8

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	A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase
	Jiang, BL; Wang, SY; Wang, YC; Chen, JH; Li, WJ; Liu, J; Hu, W; Xiao, GQ; Dong, MY; Xu, FQ
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2017-01
卷号	28 期号:1
关键词	High-throughput screening method Aspergillus niger H11 Cellulase C-12(6+) ion beam
ISSN号	1001-8042
DOI	10.1007/s41365-016-0157-8
文献子类	Article
英文摘要	In this study, a high-throughput screening method was established through the 24-square deep-well microliter plate (MTP) fermentation and micro-plate detection for large-scale screening of the mutants. It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation. Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation. The filter paper activity and beta-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23 % separately compared with A. niger H11 by shaking flask fermentation, and it was genetically stable after being passaged to nine generations. The results indicate that the high-throughput screening method can be used for the quick breeding of A. niger with high cellulase activity.
学科主题	Nuclear Science & Technology ; Physics
出版地	SINGAPORE
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [11305225] ; Science and Technology Service Network Initiative [KFJ-EW-STS-086]
语种	英语
WOS记录号	WOS:000393150100004
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/189594]
专题	学院待认领_期刊论文
通讯作者	Wang, SY (reprint author), Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China.; Wang, SY (reprint author), Lanzhou Univ, 222 South Tianshui Rd, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Jiang, BL,Wang, SY,Wang, YC,et al. A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase[J]. NUCLEAR SCIENCE AND TECHNIQUES,2017,28(1).
APA	Jiang, BL.,Wang, SY.,Wang, YC.,Chen, JH.,Li, WJ.,...&Wang, SY .(2017).A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase.NUCLEAR SCIENCE AND TECHNIQUES,28(1).
MLA	Jiang, BL,et al."A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase".NUCLEAR SCIENCE AND TECHNIQUES 28.1(2017).