Transcriptome response of wheat Norin 10 to long-term elevated CO_2 under high yield field condition | |
Lin Yuebing1; Shen Chengguo2; Lin Erda1; Hao Xingyu3; Han Xue1 | |
刊名 | Journal of Integrative Agriculture |
2016 | |
卷号 | 15期号:9页码:2142-2152 |
关键词 | Norin 10 transcriptome response wheat long term elevated carbon dioxide GO enrichment |
ISSN号 | 2095-3119 |
DOI | 10.1016/S2095-3119(16)61421-1 |
英文摘要 | The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate change by tuning its inherent molecular mechanism, which is not well understood. The present study employed the RNA-Seq method to generate transcriptome profiles of the wheat Norin 10 in response to elevated CO_2 in comparison with ambient CO_2. The 10 895 787 high-quality clean reads of Norin 10 were assembled de novo using Trinity (without a reference genome) resulting in a total of 18 206 candidate transcripts with significant BLAST matches. GO enrichment analysis of Norin 10 at different CO_2 concentrations showed that some functional genes related to plastids, precursor metabolites, and energy, thylakoid and photosynthesis were apparently enriched at elevated CO_2 (550 μmol mol~(–1)) in contrast to that at ambient CO_2 (400 μmol mol~(–1)); these findings were further confirmed by RT-PCR analysis. The findings demonstrated the specific effects of elevated CO_2 during long-term period in free air CO_2 enrichment (FACE) on transcriptome response of the high yielding wheat variety, Norin 10, which has a large spike. |
学科主题 | 农作物 |
语种 | 中文 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/101457] |
专题 | 农业环境与可持续发展研究所 |
作者单位 | 1.Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Key Laboratory of Agricultural Environment, Ministry of Agriculture, Beijing, 100081 2.Bioinformatics Unit, Source BioScience, UK, Nottingham, NG8 6PX 3.College of Agronomy, Shanxi Agricultural University, Taigu, 030801 |
推荐引用方式 GB/T 7714 | Lin Yuebing,Shen Chengguo,Lin Erda,et al. Transcriptome response of wheat Norin 10 to long-term elevated CO_2 under high yield field condition[J]. Journal of Integrative Agriculture,2016,15(9):2142-2152. |
APA | Lin Yuebing,Shen Chengguo,Lin Erda,Hao Xingyu,&Han Xue.(2016).Transcriptome response of wheat Norin 10 to long-term elevated CO_2 under high yield field condition.Journal of Integrative Agriculture,15(9),2142-2152. |
MLA | Lin Yuebing,et al."Transcriptome response of wheat Norin 10 to long-term elevated CO_2 under high yield field condition".Journal of Integrative Agriculture 15.9(2016):2142-2152. |
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