NBS Proifling Identiifes Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

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	NBS Proifling Identiifes Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans
	Zhang Kun; Xu Jianfei; Duan Shaoguang; Pang Wanfu; Bian Chunsong; Liu Jie; Jin Liping
刊名	Journal of Integrative Agriculture
	2014
卷号	13 期号:8 页码:1662-1671
关键词	potato late blight resistance gene NBS proifling broad-spectrum resistance
ISSN号	2095-3119
英文摘要	Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes, is the most efifcient approach to control late blight. Solanum demissum is a well-known wild potato species from which 11 race-speciifc resistance genes have been identiifed, however, no broad-spectrum resistance genes like RB have been reported in this species. Here, we report a novel reisistance locus from S. demissum that potentially confer broad-spectrum resistance to late blight. A small segregating population of S. demissum were assessed for resistance to aggressive P. infestans isolates (race 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11). This coupled with nucleotide binding site (NBS) proifling analyses, led to the identiifcation of three fragments that linked to the potential candidate resistance gene(s). Cloning and sequence analysis of these fragments suggested that the identiifed resistance gene locus is located in the region containing R2 resistance gene at chromosome 4. Based on the sequences of the cloned fragments, a co-segregating sequence characterized ampliifed region (SCAR) marker, RDSP, was developed. The newly identiifed marker RDSP will be useful for marker assisted breeding and further cloning of this potential resistance gene locus.
学科主题	植物保护 ; 农作物
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100584]
专题	蔬菜花卉研究所
作者单位	Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture, Beijing, 100081
推荐引用方式 GB/T 7714	Zhang Kun,Xu Jianfei,Duan Shaoguang,et al. NBS Proifling Identiifes Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans[J]. Journal of Integrative Agriculture,2014,13(8):1662-1671.
APA	Zhang Kun.,Xu Jianfei.,Duan Shaoguang.,Pang Wanfu.,Bian Chunsong.,...&Jin Liping.(2014).NBS Proifling Identiifes Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans.Journal of Integrative Agriculture,13(8),1662-1671.
MLA	Zhang Kun,et al."NBS Proifling Identiifes Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans".Journal of Integrative Agriculture 13.8(2014):1662-1671.