The maize d2003, a novel allele of VP8, is required for maize internode elongation

doi:10.1007/s11103-013-0129-x

	The maize d2003, a novel allele of VP8, is required for maize internode elongation
	Lv, Hongkun 1; Zheng, Jun 1; Wang, Tianyu 1; Fu, Junjie 1; Huai, Junling 2; Min, Haowei 2; Zhang, Xiang 1; Tian, Baohua 2; Shi, Yunsu 1; Wang, Guoying 1
刊名	PLANT MOLECULAR BIOLOGY
	2014
卷号	84 期号:3 页码:243-257
关键词	Maize Plant height Positional cloning d2003 VP8
ISSN号	0167-4412
DOI	10.1007/s11103-013-0129-x
通讯作者	Lv, Hongkun
英文摘要	The d2003 is a natural dwarf mutant from maize inbred line K36 and has less than one-third of K36 plant height with severely shortened internodes. In this study, we reported the cloning of d2003 gene using positional cloning. The results showed that there was a single-base insertion in the coding region of Viviparous8 (VP8) in d2003 mutant, which resulted in a premature stop codon. Further genetic allelism tests confirmed that d2003 mutation is a novel allele of VP8. VP8 is mainly expressed in the stem apex, young leaves, and developing vascular tissues, and its expression levels in nodes are significantly higher than that in internodes at 12-leaf stage. Subcellular localization demonstrated that the VP8 protein is localized to the endoplasmic reticulum and the N-terminal 26 amino acids (aa) of VP8 protein are essential to its localization in ER. Further transgenic experiments showed that lack of the 26 aa leads to loss of VP8 function in Arabidopsis amp1 phenotype rescue. These results strongly suggested that the N-terminal 26 aa is critical for VP8 protein localization, and the correct protein localization of VP8 in ER is necessary for its function.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000330980600001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4938]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Lv, Hongkun,Zheng, Jun,Wang, Tianyu,et al. The maize d2003, a novel allele of VP8, is required for maize internode elongation[J]. PLANT MOLECULAR BIOLOGY,2014,84(3):243-257.
APA	Lv, Hongkun.,Zheng, Jun.,Wang, Tianyu.,Fu, Junjie.,Huai, Junling.,...&Wang, Guoying.(2014).The maize d2003, a novel allele of VP8, is required for maize internode elongation.PLANT MOLECULAR BIOLOGY,84(3),243-257.
MLA	Lv, Hongkun,et al."The maize d2003, a novel allele of VP8, is required for maize internode elongation".PLANT MOLECULAR BIOLOGY 84.3(2014):243-257.