Hybrid Sterility in Rice (Oryza sativa L.) Involves the Tetratricopeptide Repeat Domain Containing Protein

doi:10.1534/genetics.115.183848

	Hybrid Sterility in Rice (Oryza sativa L.) Involves the Tetratricopeptide Repeat Domain Containing Protein
	Yu, Yang 1; Zhao, Zhigang 1; Shi, Yanrong 1; Tian, Hua 1; Liu, Linglong 1; Bian, Xiaofeng 1; Xu, Yang 1; Zheng, Xiaoming 2; Gan, Lu 2; Shen, Yumin 1
刊名	GENETICS
	2016
卷号	203 期号:3 页码:1439-+
关键词	hybrid sterility female gamete tetratricopeptide repeat (TPR) transgenic rice (Oryza sativa L.)
ISSN号	0016-6731
DOI	10.1534/genetics.115.183848
通讯作者	Yu, Yang
英文摘要	Intersubspecific hybrid sterility is a common form of reproductive isolation in rice (Oryza sativa L.), which significantly hampers the utilization of heterosis between indica and japonica varieties. Here, we elucidated the mechanism of 57, which specially causes Aus-japonica/indica hybrid female sterility, through cytological and genetic analysis, map-based cloning, and transformation experiments. Abnormal positioning of polar nuclei and smaller embryo sac were observed in F-1 compared with male and female parents. Female gametes carrying S7(cp) and S7' were aborted in S7(ai)/S7(cp) and S7(ai)/S7(i), respectively, whereas they were normal in both N22 and Dular possessing a neutral allele, S7(n). S7 was fine mapped to a 139-kb region in the centromere region on chromosome 7, where the recombination was remarkably suppressed due to aggregation of retrotransposons. Among 16 putative open reading frames (ORFs) localized in the mapping region, ORF3 encoding a tetratricopeptide repeat domain containing protein was highly expressed in the pistil. Transformation experiments demonstrated that ORF3 is the candidate gene: downregulated expression of ORF3 restored spikelet fertility and eliminated absolutely preferential transmission of S7(ai) in heterozygote S7(ai)/S7(cp); sterility occurred in the transformants Cpslo17-S7(ai). Our results may provide implications for overcoming hybrid embryo sac sterility in intersubspecific hybrid rice and utilization of hybrid heterosis for cultivated rice improvement.
学科主题	Genetics & Heredity
语种	英语
出版者	GENETICS SOCIETY AMERICA
WOS记录号	WOS:000379473600031
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4500]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 3.Nihon Univ, Coll Bioresources, Dept Plant & Resources, Fujisawa, Kanagawa 2528510, Japan
推荐引用方式 GB/T 7714	Yu, Yang,Zhao, Zhigang,Shi, Yanrong,et al. Hybrid Sterility in Rice (Oryza sativa L.) Involves the Tetratricopeptide Repeat Domain Containing Protein[J]. GENETICS,2016,203(3):1439-+.
APA	Yu, Yang.,Zhao, Zhigang.,Shi, Yanrong.,Tian, Hua.,Liu, Linglong.,...&Wan, Jianmin.(2016).Hybrid Sterility in Rice (Oryza sativa L.) Involves the Tetratricopeptide Repeat Domain Containing Protein.GENETICS,203(3),1439-+.
MLA	Yu, Yang,et al."Hybrid Sterility in Rice (Oryza sativa L.) Involves the Tetratricopeptide Repeat Domain Containing Protein".GENETICS 203.3(2016):1439-+.