Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives

doi:10.1016/j.plaphy.2015.12.001

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	Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives
	Wu, Tao 1; Yang, Chunyan 1; Ding, Baoxu 1; Feng, Zhiming 1; Wang, Qian 1; He, Jun 1; Tong, Jianhua 3; Xiao, Langtao 3; Jiang, Ling 1; Wan, Jianmin 1,2
刊名	PLANT PHYSIOLOGY AND BIOCHEMISTRY
	2016
卷号	99 页码:27-38
关键词	ABA-GA Germination Microarray Oryza sativa Seed dormancy
ISSN号	0981-9428
DOI	10.1016/j.plaphy.2015.12.001
通讯作者	Wu, Tao
英文摘要	Seed dormancy in rice is an important trait related to the pre-harvest sprouting resistance. In order to understand the molecular mechanisms of seed dormancy, gene expression was investigated by transcriptome analysis using seeds of the strongly dormant cultivar N22 and its less dormant mutants Q4359 and Q4646 at 24 days after heading (DAH). Microarray data revealed more differentially expressed genes in Q4359 than in Q4646 compared to N22. Most genes differing between Q4646 and N22 also differed between Q4359 and N22. GO analysis of genes differentially expressed in both Q4359 and Q4646 revealed that some genes such as those for starch biosynthesis were repressed, whereas metabolic genes such as those for carbohydrate metabolism were enhanced in Q4359 and Q4646 seeds relative to N22. Expression of some genes involved in cell redox homeostasis and chromatin remodeling differed significantly only between Q4359 and N22. The results suggested a close correlation between cell redox homeostasis, chromatin remodeling and seed dormancy. In addition, some genes involved in ABA signaling were down-regulated, and several genes involved in GA biosynthesis and signaling were up regulated. These observations suggest that reduced seed dormancy in Q4359 was regulated by ABA GA antagonism. A few differentially expressed genes were located in the regions containing qSdn-1 and qSdn-5 suggesting that they could be candidate genes underlying seed dormancy. Our work provides useful leads to further determine the underling mechanisms of seed dormancy and for cloning seed dormancy genes from N22. (C) 2015 Elsevier Masson SAS. All rights reserved.
学科主题	Plant Sciences
语种	英语
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号	WOS:000368954700004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4583]
专题	作物科学研究所
作者单位	1.Nanjing Agr Univ, Res Ctr Jiangsu Plant Gene Engn, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 3.Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Wu, Tao,Yang, Chunyan,Ding, Baoxu,et al. Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2016,99:27-38.
APA	Wu, Tao.,Yang, Chunyan.,Ding, Baoxu.,Feng, Zhiming.,Wang, Qian.,...&Wan, Jianmin.(2016).Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives.PLANT PHYSIOLOGY AND BIOCHEMISTRY,99,27-38.
MLA	Wu, Tao,et al."Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives".PLANT PHYSIOLOGY AND BIOCHEMISTRY 99(2016):27-38.