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	Biosynthesis and Characterization of 3-Hydroxyalkanoate Terpolyesters With Adjustable Properties by Aeromonas hydrophila
	Zhang, Hai-Feng ; Ma, Long ; Wang, Zhi-Hui ; Chen, Guo-Qiang
	2010-10-12 ; 2010-10-12
关键词	polyhydroxyalkanoates 3-hydroxyvalerate Aeromonas hydrophila PHA synthase mechanical property biopolyester ALCALIGENES-EUTROPHUS ENHANCED PRODUCTION ESCHERICHIA-COLI POLYHYDROXYALKANOATES POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE) ACID INDUSTRIAL Biotechnology & Applied Microbiology
中文摘要	Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and enoyl-CoA hydratase, and another its recombinant harboring phaAB encoding beta-ketothiolase and acetoacetyl-CoA reductase, respectively, when grown in lauric acid and/or valerate. The terpolyesters produced by A. hydrophila 4AK4 (phaAB) grown in velarate were found to produce copolymers P(3HB-co-3HV) containing high 3HV fractions with a maximum of 99 mol% 3HV. In terpolyesters, 3HV ranged from 9 to 32 mol% depending on the valerate concentration and strain used. A maximal terpolyester P(3HB-co-3HV-co-3HHx) content in dry cells was 71 wt%. Transmission electron microscopy study of A. hydrophila 4AK4 harboring phaPCJ revealed the full occupation of terpolyester P(3HB-co-3HV-co-HHx) in the cellular spaces. Terpolyesters with various monomer compositions showed changing thermal and mechanical properties. Those with higher 3HV fractions demonstrated an improved property over the lower HV containing ones. Biotechnol. Bioeng. 2009;104: 582-589. (C) 2009 Wiley Periodicals, Inc.
语种	英语 ; 英语
出版者	JOHN WILEY & SONS INC ; HOBOKEN ; 111 RIVER ST, HOBOKEN, NJ 07030 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81899]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Hai-Feng,Ma, Long,Wang, Zhi-Hui,et al. Biosynthesis and Characterization of 3-Hydroxyalkanoate Terpolyesters With Adjustable Properties by Aeromonas hydrophila[J],2010, 2010.
APA	Zhang, Hai-Feng,Ma, Long,Wang, Zhi-Hui,&Chen, Guo-Qiang.(2010).Biosynthesis and Characterization of 3-Hydroxyalkanoate Terpolyesters With Adjustable Properties by Aeromonas hydrophila..
MLA	Zhang, Hai-Feng,et al."Biosynthesis and Characterization of 3-Hydroxyalkanoate Terpolyesters With Adjustable Properties by Aeromonas hydrophila".(2010).

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