Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol

CORC > 天津工业生物技术研究所 > 功能糖与天然活性物质研究组孙媛霞 > 期刊论文

	Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol
	Yang, Jiangang; Zhu, Yueming; Men, Yan; Sun, Shangshang; Zeng, Yan; Zhang, Ying; Sun, Yuanxia; Ma, Yanhe
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2016-12-21
卷号	64 期号:50 页码:9497-9505
关键词	rare sugars aldolases Corynebacterium glutamicum metabolic engineering glycerol
英文摘要	Rare sugars are valuable natural products widely used in pharmaceutical and food industries. In this study, we expected to synthesize rare ketoses from abundant glycerol using dihydroxyacetone phosphate (DHAP)-dependent aldolases. First, a new glycerol assimilation pathway was constructed to synthesize DHAP. The enzymes which convert glycerol to 3-hydroxypropionaldehyde and L-glyceraldehyde were selected, and their corresponding aldehyde synthesis pathways were constructed in vivo. Four aldol pathways based on different aldolases and phosphorylase were gathered. Next, three pathways were assembled and the resulting strains synthesized 5-deoxypsicose, 5-deoxysorbose, and 5-deoxyfructose from glucose and glycerol and produce L-fructose, L-tagatose, L-sorbose, and L-psicose with glycerol as the only carbon source. To achieve higher product titer and yield, the recombinant strains were further engineered and fermentation conditions were optimized. Fed-batch culture of engineered strains obtained 38.1 g/L 5-deoxypsicose with a yield of 0.91 +/- 0.04 mol product per mol of glycerol and synthesized 20.8 g/L L-fructose, 10.3 g/L L-tagatose, 1.2 g/L L-sorbose, and 0.95 g/L L-psicose.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]	Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]	ESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; ACID PRODUCTION ; L-FRUCTOSE ; L-PSICOSE ; L-SORBOSE ; BIOSYNTHESIS ; DEHYDROGENASE ; ISOMERASE ; OVEREXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000390729100004
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2280]
专题	天津工业生物技术研究所_功能糖与天然活性物质研究组孙媛霞 _期刊论文
作者单位	Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Yang, Jiangang,Zhu, Yueming,Men, Yan,et al. Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(50):9497-9505.
APA	Yang, Jiangang.,Zhu, Yueming.,Men, Yan.,Sun, Shangshang.,Zeng, Yan.,...&Ma, Yanhe.(2016).Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(50),9497-9505.
MLA	Yang, Jiangang,et al."Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.50(2016):9497-9505.