Coupling of small leucine-rich proteoglycans to hypoxic survival of a progenitor cell-like subpopulation in Rhesus Macaque intervertebral disc

	Coupling of small leucine-rich proteoglycans to hypoxic survival of a progenitor cell-like subpopulation in Rhesus Macaque intervertebral disc
	Huang, Shishu; Leung, Victor Y. L.; Long, Dan; Chan, Danny; Lu, William W.; Cheung, Kenneth M. C.; Zhou, Guangqian
刊名	BIOMATERIALS
	2013
英文摘要	Degeneration of the intervertebral disc (IVD) is a major spinal disorder that associates with neck and back pain. Recent studies of clinical samples and animal models for IVD degeneration have identified cells with multi-potency in the IVD. However, IVD tissue-specific progenitor cells and their niche components are not clear, although degenerated IVD-derived cells possess in vitro characteristics of mesenchymal stromal cell (MSCs). Here, we firstly identified the tissue-specific intervertebral disc progenitor cells (DPCs) from healthy Rhesus monkey and report the niche components modulated the survival of DPCs under hypoxia. DPCs possess clonogenicity, multipotency and retain differentiation potential after extended expansion in vitro and in vivo. In particular, the nucleus pulposus-derived DPCs are sensitive to low oxygen tension and undergo apoptosis under hypoxic conditions due to their inability to induce/stabilize hypoxia-inducible factors (HIF). The presence of small leucine-rich proteoglycans (SLRP), biglycan or decorin, can reduce the susceptibility of DPCs to hypoxia-induced apoptosis via promoting the activation/stabilization of HIF-1 alpha and HIF-2 alpha. As IVD is avascular, we propose SLRPs are niche components ofDPCs in IVD homeostasis, providing new insights in progenitor cell biology and niche factors under a hypoxic microenvironment. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S0142961213005929
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/5198]
专题	深圳先进技术研究院_医药所
作者单位	BIOMATERIALS
推荐引用方式 GB/T 7714	Huang, Shishu,Leung, Victor Y. L.,Long, Dan,et al. Coupling of small leucine-rich proteoglycans to hypoxic survival of a progenitor cell-like subpopulation in Rhesus Macaque intervertebral disc[J]. BIOMATERIALS,2013.
APA	Huang, Shishu.,Leung, Victor Y. L..,Long, Dan.,Chan, Danny.,Lu, William W..,...&Zhou, Guangqian.(2013).Coupling of small leucine-rich proteoglycans to hypoxic survival of a progenitor cell-like subpopulation in Rhesus Macaque intervertebral disc.BIOMATERIALS.
MLA	Huang, Shishu,et al."Coupling of small leucine-rich proteoglycans to hypoxic survival of a progenitor cell-like subpopulation in Rhesus Macaque intervertebral disc".BIOMATERIALS (2013).