A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079 | |
Chan, Zhuhua; Zhong, Tianhua; Yi, Zhiwei; Xiao, Jing; Zeng, Runying | |
刊名 | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING |
2015 | |
卷号 | 20期号:5页码:908-914 |
关键词 | Streptomyces sp MC079 enduracidin pH-shift feeding strategy fed-batch fermentation |
通讯作者 | zeng@tio.org.cn |
中文摘要 | The aim of this work is to enhance enduracidin production by Streptomyces sp. MC079. Based on the time course analysis of the specific cell growth rate and specific enduracidin formation rate, a two-stage pH control strategy was proposed to improve enduracidin production by shifting the culture pH from 5.5 to 5.8 after 112 h of cultivation. By applying this pH control strategy, enduracidin concentration and productivity was 51.2 and 65.0% higher than results with uncontrolled pH batch fermentation. For further enhancement of enduracidin production, the effects of constant-rate feeding and pH-shift feeding strategy were investigated. The results indicated that the pH-shift feeding strategy increased the maximum concentration and productivity of enduracidin to 61.37 mg/L and 0.697 mg/L/h in the constant-rate feeding fermentation process. This is 73.3 and 88.9% higher than results with uncontrolled pH batch fermentation, respectively. The obtained optimal pH shift feeding strategy may be useful for the industrial-scale microbial production of enduracidin. |
学科主题 | Biotechnology & Applied Microbiology |
收录类别 | SCI |
内容类型 | 期刊论文 |
源URL | [http://ir.scsio.ac.cn/handle/344004/15029] |
专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
推荐引用方式 GB/T 7714 | Chan, Zhuhua,Zhong, Tianhua,Yi, Zhiwei,et al. A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2015,20(5):908-914. |
APA | Chan, Zhuhua,Zhong, Tianhua,Yi, Zhiwei,Xiao, Jing,&Zeng, Runying.(2015).A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,20(5),908-914. |
MLA | Chan, Zhuhua,et al."A pH shift feeding strategy for increased enduracidin production during fed-batch fermentation by a deep-sea, bacterium, Streptomyces sp MC079".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 20.5(2015):908-914. |
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